Is the Total in the Sub Form Footer or the Parent Form Footer?

It is in both but not visible on the sub form footer.

The totals on the parent form are being saved to the order table.

How are you letting Parent Form know that the SubForm has been updated?

The totals refresh fine when changing the quantity manually.

I've also tried me.parent.requery and me.parent.refresh in the after update for the subform and it still doesn't refresh unless I go in manually and change the quantity.

What does the barcode scanning do?
Which field does it change?

When I scan an item it adds it to the order and refreshes both the sub form and parent form footer totals, but when I scan the same item again it updates the quantity and refreshes the sub form footer totals but the parent form footer totals do not refresh. I even clicked the refresh button and it still doesn't refresh the totals in the parent form. Attached is the form with the 2 different totals after scanning the same item 2 times. Also attached it the code I used for the barcodes scanning to increment the quantity.

Instead of using VBA code to update the parent form's totals, use references of the subform controls containing the totals instead.  In each of the parent form's total textboxes, go to Properties -> Control source, enter something like this:

     =[Put subform's name here].[Form]![Put subform's total textbox name here]

This way, the parent's textboxes will always show the subform's textboxes' values, always.  No refreshing is needed, because this is a *direct reference* that will always show the value the reference is pointing to.

The reason AfterUpdate only works when you enter data manually is because that is exactly how AfterUpdate works.  If you use code to change form data, Access doesn't see that as an "update" and won't run AfterUpdate.  This is what Microsoft's documentation ( https://learn.microsoft.com/en-us/office/vba/api/access.form.afterupdate-event ) says: "Changing data in a control by using Visual Basic or a macro ... doesn't trigger these events..."

Thank Kevin, I had that in the totals control box prior, but took it out so I could save it to my table. It does work but know my totals are not saved to the order table.

Maybe you don't need to save the totals.  Totals are going to be changed all the time, so it doesn't make sense to have to save them every time.  The general rule of thumb is that anything that needs to be calculated shouldn't be saved to a table -- they need to be obtained via calculations instead (in queries, calculated fields, etc.).